I am trying to mine data acquired from high content imaging. The basic idea of the analysis is that I would like to first plot the mean intensity of a nuclear label vs the size of the nuclei, this effectively allows one to look at the cell cycle. I then want to select a certain area on the XY chart and filter the data. I can/have done this successfully with the data connector; however, I am not satisfied with only being constrained to selecting a box. Is there a way to select an odd shape region on an interactive XY chart, or is SHIFT + box what I am stuck with? Next, how can I pass the selected data to calculate a mean value of another property and plot this in real time?

I have included a data set and an initial attempt at the protocol. The green area on the second XY chart is what I want to select (I selected this by coding in ranges to give a general Idea of the region I want to save). The properties in the data I used are INTENSITY_nuclear_mask_ch00_PixelIntensityMean and SHAPE_nuclear_mask_BlobArea for my initial filters. This then highlights the cells on a histogram, INTENSITY_nuclear_mask_ch00_SumOfPixelIntensities, to confirm cell cycle location (These cells are mostly synchronized so only one major peak should come out). Finally, I  would like to take the mean of BOOLEAN_ObjectFound ~ PlateID. Any comments or suggestions would be greatly appreciated.

Thanks,

Reid