Will an under defined sub assembly make the top level assembly under defined?

I have a full assembly with all assembly sketches and components fully defined (no minus signs next to anything), yet it still says the top level assembly is under defined.

I do have a sub assembly with concentric mates that are still able to rotate but that is it.

*Edit: I found the '(-)' sign next to several of my mirrored components. I assumed these would be defined as they are mirrored components but apparently not!