Now, I want to define a binding site of a protein. According to the literature, the Residues constituting the binding site have been known. In the Hierarchy View, the Residues been known were selected. Then Tool-Binding Site-Define Sphere from Selection, to define the binding site. However, Input Binding Site was always "Error: Could not find Binding Site". So, the docking by Ligandfit couldn't been done.
I want to know if my method of definition of binding site was wrong. I hope to learn the correct way to definite the binding site of which the Residues have bben known. Thanks!