Hi All,

I am a regular user of BIOVIA Discovery Studio and have been using the software for quite some time. I have a general query regarding the parameters used for identifying non-bonded interactions in protein–ligand complexes.

Specifically, I would like to know the default distance and angular criteria used by Discovery Studio for classifying common non-bonded interactions such as:

• Hydrogen bonds (e.g., donor–acceptor distance and angle range)
• Pi–Pi interactions (e.g., centroid–centroid distance, interplanar angle)
• Pi–cation, Pi–anion, salt bridges, and hydrophobic contacts, among others.

I have reviewed the software manual and the Help section thoroughly but could not find this specific information. I would greatly appreciate it if someone could point me to the relevant documentation or provide these parameters directly.

Thank you for your support.

Best regards,
Biswayan Ghosh