Whenever I use this protocol ,I always get 0 refined pose although the pharmacophore matching is perfect.

Here is a simple trial which I performed today.

1-I used the 1fvv protein and ligand which are found in DS sample folder

2-I autogenerated a pharmacophore using receptor ligand pharmacophore generation module

3-I removed the execluded volumes

4-I run the charmm with pharm.constrain modules using the defaults except that I set conf generation to none as this was the bound conformer

5-The result is always the same whatever the conditions

0 refined pose

I tried these options too but in vain

a) setting the force restraint to lower value

b)setting simulated annealing to none

Please help me out

I tried to use cdocker on the conformer obtained from pharmacophore alignment and set random conformations to 0 and it gave good poses