I am using Discovery Studio 2.1 with CFF, I have an enzyme that binds a substrate in a disordered region, on both sides of the disordered substrate there are globular domains that loop around the enzyme and make interactions that stabilize the looped substrate and so prevent the enzyme from dissociating from the substrate. I can calculate the interaction energy between the enzyme and bound substrate, but how can I quantify the affect the looped substrate has on preventing the enzyme from dissociating from the substrate? The looped substrate globular domains do not make direct interactions with the enzyme. Thank you for any suggestions