Hy @ all,
I'm trying to build a homology model with discovery studio.
What I've done allready:
1. load my Model Sequence
2. used Tool Search Sequences by Similarity, then I found one good template
3. load Structure and Alignment for this template
4. used tool Align Sequences to Templates
5. used tool build homology model
then I' m getting this error message
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at /home/sbg-acc-user/acc-server/apps/scitegic/dscore/server/lib/vendor_perl/5.8.9/ScriptObject.pm line 82
ScriptObject::DeferredCall::___CallRightValueContext('ScriptObject::DeferredCall=SCALAR(0x784d2e0)', 'ARRAY(0x784d4e0)') called at /home/sbg-acc-user/acc-server/apps/scitegic/dscore/server/lib/vendor_perl/5.8.9/ScriptObject.pm line 136
ScriptObject::DeferredCallTie::FETCH(undef) called at /home/sbg-acc-user/acc-server/apps/scitegic/dscore/lang/perl/Components/SuperimposeProteins.pm line 195
ScriptObject::Object::AUTOLOAD('ScriptObject::Object=HASH(0x15e0acc0)', 'ScriptObject::Object=HASH(0x784d6d0)', '-------------------------------------------------PEPLSQQWTAGM...', 'ScriptObject::Object=HASH(0x784d730)', '-------------------------------------------------PEPLSQQWTAGM...', 'False') called at /home/sbg-acc-user/acc-server/apps/scitegic/dscore/lang/perl/Components/SuperimposeProteins.pm line 195
main::UpdateRMSDList('ScriptObject::Object=HASH(0x15e0acc0)', 'ScriptObject::ObjectArrayCollectionTie=ARRAY(0x14fce180)', 'ScriptObject::ObjectArrayCollectionTie=ARRAY(0x1412bec0)', 0, 1, '', 'ARRAY(0x15e0b110)', 'superimposeProteinUsingBackboneAtoms', 'ARRAY(0x2d7bc90)', ...) called at /home/sbg-acc-user/acc-server/apps/scitegic/dscore/lang/perl/Components/SuperimposeProteins.pm line 459
main::onProcess
Error calling function
Error during call to function pilot::setup::scriptingOnProcess
PerlScript::HandleError: Pipeline Pilot exception rethrown
PerlScript::Call: Pipeline Pilot exception rethrown
CComponentPerlPlugin::onProcess: Pipeline Pilot exception rethrown
-> Perl (on Server) (Perl (on Server)) - error during Data Record Processing phase
CProtocolStd::onProcess: Pipeline Pilot exception rethrown
CProtocol::onProcess: Pipeline Pilot exception rethrown
-> Superimpose Proteins by Alignment (Superimpose Proteins by Alignment) - error during Data Record Processing phase
CProtocolStd::onProcess: Pipeline Pilot exception rethrown
CProtocol::onProcess: Pipeline Pilot exception rethrown
-> Superimpose Templates and Models (Subprotocol) - error during Data Record Processing phase
CProtocolStd::onProcess: Pipeline Pilot exception rethrown
CProtocol::onProcess: Pipeline Pilot exception rethrown
-> Save Homology Models (Save Homology Models) - error during Data Record Processing phase
CProtocolStd::onProcess: Pipeline Pilot exception rethrown
CProtocol::onProcess: Pipeline Pilot exception rethrown
CComponentRunner::CallOnProcess: exception caught, calling onFinalize before rethrow
Protocol Build Homology Models, user praktikant: Pipeline Pilot exception caught
Protocol Build Homology Models, user praktikant: Protocol failed: Pipeline Pilot error
Pipeline Pilot Server version 8.5.0.200
Server OS: Linux ardmore 2.6.32-5-amd64 #1 SMP Mon Sep 23 22:14:43 UTC 2013 x86_64 GNU/Linux
Have anyone an ides, how I can handle this?
Thanks