Hello.

I'm a new user. I did a protein docking and i wanna know how much binding energy between two protein I docked. I tried to use Calculate Binding Energy protocol, the ligan should saved as *.sd file and  I have the charge to convert in integer. Unfortunately, its failed. Do the Calculate Binding Energy protocol use for small molecule ligand only? How do I calculate the binding energy and binding affinity between two protein?

Thank you in advance.

Regards

Herta