Fluid subdomains or initial condition same behaviour ?

Dear readers,

I'am simulating a closed model witch is divided in to two sections.
One is filled with air one with an user defined liquid. ( divided by a lid )

I difined two fluid subdomains, which are previewed and shown blue when i click one lid.

I also see the function initial condition where i need to select all the faces which are in contact with the fluid.

What is the diffrence between initial conditions and fluidsubdomains, do i need to set both or does the simulation works fine like i did ?
( Simulation takes ages so i cant just test it )

Thanks in advance !

Koen

SolidworksFlow Simulation